Development of an innovative hydrogel printing head for printing osteochondral structures
Loading...
Date
2024-11-11
Authors
Φανίδης, Βασίλειος
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In recent years, 3D bioprinting has been used in tissue engineering, addressing the limitations 2D cultures have. This technology is not only used for research but also to meet the growing need for engineered tissue constructs. This study presents the design and evaluation of a novel Klipper-operated bioprinting head, “MyPrintHead,” capable of printing bone and myeloma structures using GelMA hydrogel (also capable of printing any cell-laden hydrogel), and all the
modifications (software and hardware) made to alter an FDM printer to a Klipper bioprinter. Comparison of “MyPrintHead” with a literature-established printhead, “Replistruder4”, using evaluation of cost and weight, usability, functionality, and cell viability assays in SAOS-2 and NCI-H929 cell lines resulted in differences in all the above-mentioned aspects of the two compared heads. In addition, modification of “Replistruder4” was carried out to optimize its defects, so that it can be used in this specific research process successfully. Overall, “MyPrintHead” turned out to be better as it presented higher cell viability rates and required fewer recalibrations due to its reduced weight. These findings suggest that “MyPrintHead” offers a cost-effective, reliable solution for hydrogel 3D bioprinting applications in tissue engineering.
Description
Keywords
Printhead, bioprinting head, Luer lock syringe, Replistruder4-modified, MyPrintHead, GelMA, SAOS-2, NCI-H929, Osteosarcoma, Multiple myeloma, Hydrolysis