Characterization of an HSA column through HPLC : testing the Abraham solvation model
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Date
2024-07-12
Authors
Χαλούλου, Παναγιώτα
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Abstract
High-pressure liquid chromatography (HPLC) is a powerful technique for separating sample components based on their interactions with the mobile and stationary phases, which vary according to the molecules' affinities with the adsorbent surface. This method has revolutionized pharmaceutical analysis, significantly advancing drug discovery and development by reducing costs and enabling early selection of promising molecules. Over the past two decades, HPLC analysis using protein-packed columns, such as Human Serum Albumin (HSA), has provided insights into drug metabolism and pharmacokinetics based on their molecular structure and physicochemical properties. Characterizing such a system involves determining the column's hold-up time and solute-solvent interactions, often using Abraham’s solvation parameter model. A new fast method has been recently proposed to yield similar results in significantly less time. Despite challenges in matching this new fast method to the model, results indicate that cavity formation and hydrogen bond donor interactions govern retention behavior in this reversed-phase system. These findings underscore HPLC's value in rapidly and accurately assessing drug-protein interactions, promising significant advancements in pharmaceutical research.
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Keywords
HSA, HPLC, Abraham solvation parameter model